Sidik toxoplasma crispr screen
WebAuthors: Saima M Sidik, Diego Huet & Sebastian Lourido Apicomplexan parasites, such as Toxoplasma gondii, cause extensive morbidity and mortality in humans and livestock, … WebJun 27, 2014 · Citation: Sidik SM, Hacke tt CG, Tran F, Westwood NJ, Lourido S (2014) Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9. PLoS ONE 9(6): e100450. doi:10.1 371/journal.pone.0100450
Sidik toxoplasma crispr screen
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WebThe following articles are merged in Scholar. Their combined citations are counted only for the first article. WebA CRISPR/Cas9 gene editing strategy has been remarkable in excising segments of integrated HIV-1 DNA sequences from the genome of latently infected human cell lines …
WebDOI: 10.1016/j.chom.2024.09.008 Corpus ID: 204244046; In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration. WebOct 16, 2024 · Using a genome-wide CRISPR screen we identify 353 Toxoplasma genes that determine parasite fitness in naїve or IFNγ-activated murine macrophages, seven of which are further confirmed. We show that one of these genes encodes dense granule protein GRA45, which has a chaperone-like domain, is critical for correct localization of GRAs into …
WebWe recently used CRISPR-Cas9 to disrupt all nuclear protein–coding genes in T. gondii using a pooled format. The method relies on transfection of a guide RNA library into parasites … WebAug 13, 2024 · The Lourido laboratory at the Whitehead Institute has led the charge of applying the power of CRISPR screens to Toxoplasma ... Overcoming initial hurdles of …
WebThe integrated sgRNAs can be used as barcodes to measure the contribution of targeted genes to cell fitness. Despite the lack of viral transduction, we adapted CRISPR/Cas9 for …
WebSep 1, 2016 · Saima Sidik, who co-authored the study and is a research assistant in the Lourido lab agrees, ... A genome-wide CRISPR screen in Toxoplasma identifies essential … solar flickering candle lanternWebIn Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration. by Lamba Omar Sangaré, Einar B Ólafsson, Yifan Wang, Ninghan Yang, Lindsay … slump meaning scienceWebDOI: 10.1016/j.xpro.2024.100520 Corpus ID: 234778481; CRISPR screen to determine the in vivo fitness of Toxoplasma genes @article{Sangar2024CRISPRST, title={CRISPR screen to determine the in vivo fitness of Toxoplasma genes}, author={Lamba Omar Sangar{\'e} and Yifan Wang and David Arranz-Sol{\'i}s and Jeroen P. J. Saeij}, journal={STAR Protocols}, … solar flickering window candlesWebWe recently used CRISPR-Cas9 to disrupt all nuclear protein-coding genes in T. gondii using a pooled format. The method relies on transfection of a guide RNA library into parasites … slump of concrete as per is codeWebIn this study, Sidik et al. present the first genome-wide knockout screen performed in T. gondii. To do so, they adapted the CRISPR/Cas9 system to screen pools of transgenic … solar flickering flame pathway lightsWebBy performing a genome-wide CRISPR screen in the apicomplexan Toxoplasma gondii, the lab of Whitehead Fellow Sebastian Lourido was able to identify previously … solar flickering flame outdoor lightsWebA new preprint by the Lourido lab uses CRISPR screens to find genes that sensitize Toxoplasma to an antimalarial drug, aiming to shed light on resistance in the related malaria parasite. The study ... solar flieger watch