WebMitotic kinetochores are initially captured by dynamic microtubules via a ‘search-and-capture’ mechanism. The microtubule motor, dynein, is critical for kinetochore capture as it has been shown to transport microtubule-attached chromosomes towards the spindle pole during early mitosis. The microtubule-binding Ndc80 complex that is recruited to … WebImageJ (National Institute of Mental Health, Bethesda, MD, USA) densitometry software was used for quantification of Western blot bands. Selected bands were quantified based on their relative integrated intensities, calculated as the product of the selected pixel area and the mean gray value for those pixels normalized to the internal control.
Coordination between the Ndc80 complex and dynein is essential …
Web14 mrt. 2024 · There are two approaches to normalization when evaluating a western blot: single protein detection and total protein normalization. By far the most common approach to single protein detection is to use housekeeping proteins. Their abundance within a sample serves as a proxy for the entire protein population. Web21 jan. 2024 · These findings apply to all Western blot studies, and we highlight quality control checks that should be performed to make Western blot data more quantitative. 1. Introduction Western blotting is routinely used to detect proteins and their posttranslational modifications (PTM) in biological samples. pack of underwear
Quantitative Analysis of Histological Staining and Fluorescence …
Web29 jun. 2024 · Introduction. Kidney stone disease, which occurs in nearly 9% of adults and continues to increase in prevalence worldwide [1, 2], is a risk factor for chronic kidney disease (CKD) [3, 4] and may still cause death in the 21st century [].Although many studies have focused on the prevention of kidney stone formation, no effective medical treatment … WebWestern Blot Image Analysis: Lane and Band Tools. The other common approach for quantitating western blots is commonly referred to as using lane and band tools. This … WebWestern blot quantification method constitutes a critical step in order to obtain accurate and reproducible results. Due to the technical knowledge required for densitometry … jerrod goff wife