Hepg2 electroporation
WebElectroporation is a physical method for cell transfection, where application of high voltage electric pulses is used to transiently permeabilize cell membranes allowing … Web14 okt. 2024 · USP8 knockdown facilitates ferroptosis induced by erastin via ferritinophagy. (A) Knockdown of USP8 in MEF cells was verified by immunoblotting. (B) MEF cells were treated with indicated erastin for 24 h, cell viability was assayed using a CCK8 kit (n = 3).(C) MEF cells were treated with 0.5 μmol/L or 1 μmol/L erastin for 12 h, cell death was …
Hepg2 electroporation
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WebPassionate, motivated, and energetic scientist with over 13 years of pharmaceutical research and support experience seeking to utilize and expand extensive technical skills and knowledge to ...
WebTo make stably NTCP overexpressed HepG2, I used G418 selection vector, pcDNA3.1 (+). Three days after transfection NTCP-FLAG-pcDNA3.1 (+) to HepG2, I conducted … WebThe exosomes harvested from these 293T cells were loaded with miR-26a via electroporation. Results: The engineered exosomes were shown to bind selectively to HepG2 cells via the scavenger receptor class B type 1–Apo-A1 complex and then internalized by receptor-mediated endocytosis. The release of miR-26a in exosome …
WebAdd 1μg of plasmid DNA to the medium, and mix. For a 3:1 ViaFect™ Transfection Reagent:DNA ratio, add 3μl of ViaFect™ Transfection Reagent, and mix immediately. Incubate the ViaFect™ Transfection Reagent:DNA mixture for 5–20 minutes at room temperature. Optional: Add mixture to cells without an incubation period. WebHep G2 Human hepatocellular carcinoma cell line Cell Type: Liver Epith. Tissue Origin: liver Species: human Research Area: Cancer Research/Cell Biology Gastroenterology Toxicology Cell Characteristics: Adherent Transfection Information Lonza Optimized Protocol Optimization Guideline Filter:
WebFirst, we tried to optimize the transfection of the hepatoma cell line HepG2 with PEX5 siRNA using different commercially available liposomal and non-liposomal transfection reagents …
Web12 apr. 2024 · For CHO-K1 and HepG2 cells, the frequencies of EGFP-positive cells were high about 9.10–18.8%, with no significance among different reporter forms (Figure 3A,B). Even higher, up to 49.4%, EGFP-positive cells appeared in HepaRG cells with circular reporter plasmid, which was comparable with that in the EGFP-N2 positive control group … little bit of love dog rescue tucsonWebftsj2, a heat shock-inducible mitochondrial protein, suppresses cell invasion and migration little bit of love tom grennan genreWebIn vivo electroporation has been shown to be effective for a variety of tissues, including tumors, skin, liver, lung, kidney, thymus, bladder, adipose tissue, vasculature, retina, cornea, ciliary muscle, brain, the umbilical cord of the spinal cord, skeletal muscle, and testis. little bit of love - jack johnsonWeb17 nov. 2014 · HepG2 cells accumulated higher level of BBR for longer duration than the other two cell lines. ... This P-gp promoter fused GFP construction was verified by sequencing and subjected to transfect into HepG2, HeLa and SY5Y cells by electroporation separately. Cells in serum-free medium containing 20 µg DNA were … little bit of love torrentWebsequencing and subjected to transfect into HepG2, HeLa and SY5Y cells by electroporation separately. Cells in serum-free medium containing 20 mg DNA were electroporated in an electroporator (Model ECM 630, BTX). The electroporation parameters set were 250 V voltage (low voltage model), 1575 V resistance and 1000 mF … little bit of love lyrics keshaWebThree commonly used transfection techniques (electroporation, calcium phosphate precipitation and scrape loading) and a novel procedure combining the latter two methods were evaluated and conditions optimised for successful transfection of human HepG2 cells with plasmid DNA incorporating a mouse MHC Class I gene and a selectable marker … little bit of love jp cooper lyricsWeb1 jul. 2024 · Electroporation, which uses pulsed electrical fields, can be used to introduce DNA into a variety of animal cells, plant cells, and bacteria. Electroporation works well … little bit of love tlumacz