WebThe RQ1 DNase digestion capacity was demonstrated by the treatment of free CpG-ODN (line 6). BSA in the formulation did not affect the CpG-ODN protection (lines 9 and 10). CpG-ODN, CpG-oligodeoxynucleotides; BSA, bovine serum albumin; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis. RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO 4, 10mM CaCl 2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C]) Applications
RQ1 RNase-Free DNase, 1,000u - Promega
WebRQ1 RNase-Free DNaseは、高純度に精製されたDNase I 製品で、一本鎖あるいは二本鎖DNAを分解して3'-末端に水酸基を持つオリゴヌクレオチドを生成します。 特にRNAの … WebUsing TURBO™ DNase DNase I is commonly used to clear DNA contamination from RNA samples prior to RT-PCR. Conventional DNase I has a poor affinity for DNA and cleaves … agenzia delle entrate di chiari appuntamento
RQ1 RNase-Free DNase Protocol - Promega Corporation
WebRQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO 4, 10mM CaCl 2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C]) WebJan 20, 1998 · DNase (RQ1 RNase-free DNase, Promega) was tested by using 0.025 units/μg DNA. RNase I (Promega) was tested by digesting 10 μg of RNA with 0.65 units of RNase I for 1 h. Changes in the activity of nucleic acid-cleaving or –degrading enzymes were followed by agarose electrophoresis and densitometric analysis of the intensity of … WebRQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and single-stranded DNA endonucleolytically, producing 3´-OH oligonucleotides. RQ1 RNase-Free DNase is used in applications where maintaining the integrity of the RNA is critical. This DNase is used for applications such as nick translation, production of ... agenzia delle entrate di competenza milano