WebPurpose: We established primary cultures of hIMCD (human papillary collecting duct epithelial) cells. Materials and methods: Tissues were digested enzymatically. Cells were maintained in Dulbecco's modified Eagle's medium … WebDec 22, 2024 · The expression of Cenpf was found upregulated in collecting duct cells from Pkd1 HOMO kidneys compared to that in age-matched Pkd1 HET kidneys at a single-cell resolution (Figure 3a), and this was confirmed by immunofluorescence staining with Cenpf antibody and co-staining with collecting duct cell marker, DBA (Figure 3b).
Primary culture and characterization of human renal inner …
WebWe studied the distribution of specific renal collecting duct cell markers using morphological, immunohistochemical and biochemical procedures. Cultured renal … WebSep 12, 2024 · Kidney sections were co-stained with LTA (proximal tubules marker) or DBA (collecting ducts marker), anti-PS antibody (antibody labels RGLS4326) and DAPI. Representative merged... founders brewery centennial room
Incidence, Clinical Characteristics, and Survival of …
WebMay 4, 2024 · The present study demonstrates that the transcriptional regulator, p63, is a specific marker of Ret + UBTC, which are the progenitors of all cell types of the mature collecting duct. Interestingly, expression of p63 in the UB tip is transient, beginning around E15 and ending at P5, thus coinciding temporally with the initiation of specification ... WebDifferential diagnosis of collecting duct carcinoma (CDC) from invasive upper tract urothelial carcinoma (UTUC) can be challenging. PAX8 and p63 are 2 markers often used in this setting. GATA binding protein 3 (GATA3) is a marker of urothelial differentiation. We investigated GATA3 expression in CDC and UTUC and its use in this differential. WebConnection of ES Cell-derived Collecting Ducts and Ureter-like Structures to Host Kidneys in Culture. May Sallam a Deanery of Biomedical Science ... (PKC), and the epithelial marker Krt8 stain of the grafted kidney showing a connected lumen between the eUB graft and the collecting duct branches of the host kidney (arrow), early nephrons ... founders brewery detroit parking