WebMAZ ( Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. WebWe recommend Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) ( ab6729) secondary antibody. Flow Cyt. Use 1µg for 10 6 cells. ab170190 and ab81216 - Mouse monoclonal …
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WebFeb 1, 2024 · Y1H, ChIP, EMSA, and transient expression analysis further revealed that PIF4 can specifically bind to the G-box motif in the PAP1 promoter, indicating that PIF4's inhibitory effect on PAP1 transcription is direct (Figs. S10 and 6). WebAmit Paul, Nicole C. Riddle, in Epigenetics Methods, 2024. 2.1.1 Electrophoretic mobility shift assay (EMSA). The electrophoretic mobility shift assay or EMSA is a routinely used …
WebBuffer. PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic. c-Jun antibody tested by supershift. Detection of c-Jun … WebJun 11, 2024 · The regulatory mechanisms of ERRγ and FOXM1 were analyzed by ChIP, EMSA, and siRNA. The effects of EZH2 inhibitor (GSK126) or/and ERRγ agonist (DY131) on the tumorigenesis of gastric cancer cell lines were examined by cell proliferation, transwell migration, wound healing, and colony formation assays. Meanwhile, the …
WebFeb 2, 2024 · ChIP, EMSA, and reporter gene assays showed that KLF2 could directly bind to the P1 promoter regardless of methylation status and reduced the P1 promoter activity. Consistently, histone modification analysis showed that H3K9me2 was enriched and H3K27ac was depleted in the P1 promoter upon KLF2 overexpression in ICP1 cells. … WebSep 29, 2024 · ChIP, EMSA and dual luciferase reporter assays were conducted to identify the binding sites of target genes. Co-IP, RIP, MeRIP, RNA-FISH and ubiquitin assays …
WebSep 23, 2024 · The locations of amplicons for ChIP–qPCR analysis in b and probes for EMSA experiments in f and g are also shown. b, ... ChIP methods were described previously with minor modifications 48. For ...
WebDec 14, 2024 · ChIP and EMSA were then performed, which indicated that all the randomly selected TFs were genuine upstream regulators of the AtCAT3 promoter. The use of the transiently transformed plants equally ... irmi service of suitWebApr 14, 2024 · Top 25 global and Chinese chip companies ranked Apr. 3, 2024, According to a report recently released by Gartner, global semiconductor revenue in 2024 increased by 0.2% year-on-year to $599.1 billion. irmi property insuranceWebGel Shift Assays–EMSA. The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, … The handbook provides background, helpful hints and troubleshooting advice for … irmi technology e\\u0026oWebJan 1, 2024 · Thus, various techniques such as electrophoretic mobility shift assays (EMSA), Förster resonance energy transfer (FRET), and DNA foot printing assays are employed commonly to understand the protein-DNA interaction in vitro and complement ChIP-based methods [23]. In this section, we briefly outline the methods that were … port in chest cancerWebJan 28, 2015 · The handbook provides background, helpful hints and troubleshooting advice for immunoprecipitation and co-immunoprecipitation assays, pull-down assays, far-western blotting and crosslinking. The handbook also features an expanded section on methods to study protein–nucleic acid interactions, including ChIP, EMSA, and RNA EMSA. port in chest for dialysisWebExpert Answer. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assays are two different techniques of characterizing protein … port in chest wallWebDec 18, 2024 · EMSA assay was performed according to the manufacturer’s protocol of the LightShift Chemiluminescent EMSA Kit (Life Technologies). Chromatin … port in chennai